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Immunogenicity evaluation of MS2 phage-mediated

cultured in LB medium supplemented with 50 mg/ml kanamycin. Until the OD 600 reached 0.8, target proteins were induced by 0.3 mM isopropyl -D-thiogalactoside (IPTG). After induction at 20 C for 16 h, the cells were harvested by centrifugation at 6,000 g for 15 min at 4 C. The cell pellet was re-suspended in PBS buffer and sonicated seven times Lowe's Home ImprovementCelebrate Lowes first SpringFest event a festival of fun and savings for your home and garden. Get deals on mulch, soil, power equipment, and more.

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